Role of Ureaplasma spp.in infection
My primary research interest focuses on the pathogens Ureaplasma parvum and Ureaplasma urealyticum. Ureaplasma spp. have some of the smallest genomes of free living bacteria (0.75 – 0.95 Mbp) making for an excellent model to understand the minimum complement of genes required for pathogenicity.
Ureaplasma spp. are the most commonly isolated organism from the chorioamnion among patients presenting with preterm labour and have been associated with numerous choronic infections such as chronic lung disease of prematurity, non-gonococcal urethritis (NGU) and infectious arthritis.
Treatment of these infections is complicated due to a lack of effective antibiotics. The absence of a cell wall renders Ureaplasma spp. intrinsically resistant to all beta lactam and glycopeptide antibiotics with only tetracyclines, quinolones and macrolides deemed as effective. Treatment options are further restricted in neonates due to the contraindication of tetracyclines and quinolones due to associated toxicities. A third confounding factor in treatment is the presence of antibiotic resistant strains.
My research interests include
• Monitoring the prevalence of antibiotic resistance among UK Ureaplasma isolates
• Identifying the mechanisms of antibiotic resistance
• Understanding the role of U. urealyticum and U. parvum in disease among patients with preterm labour, chronic lung disease of prematurity and nongonococcal urethritis.
Invertebrate models of infection
As part of my post-doctoral work I became interested in the use of invertebrates as a model for infection. Compared with mammalian studies this system is cost effective, ethics free and does not require specialised housing facilities. These factors make the invertebrate model a feasible intermediate between in vitro studies and larger scale in vivo mammalian work. I have successfully used these models to examine bacterial virulence factors as well to test novel anti-invectives therapies.
1. Nollmann F. I, Heinrich A. K, Brachmann A. O, Morisseau C., Mukherjee K, Casanova-Torres A. M, Kinski S, Schultz K, Beeton M. L., Kaiser M, Chu Y, Ke L, Thanwisai A, Bozhüyük K. A. J., Chantratita N, Götz F, Waterfield N. R., Vilcinskas A., Goodrich-Blair H., Hammock B. D. and Bode H. B. 2015. A Photorhabdus natural product inhibits insect juvenile hormone epoxide hydrolase. ChemBioChem. In Press
2. Beeton M. L., Aldrich-Wright. J. & Bolhuis A. 2014. The activity of copper(II) complexes against biofilms of methicillin-resistant Staphylococcus aureus. Journal of Inorganic Biochemistry. 140:167-72
3. Beeton M. L. Atkins D. & Waterfield N. R. 2013. Filamentous phenotype of Bacillus spp. in association with the bacterivorous amoeba Acanthamoeba polyphaga. Journal of Insect Pathology. 59(2): 223-31
4. Yang G., Hernández-Rodríguez C.S., Beeton M. L., Wilkinson P., Ffrench-Constant R. and Waterfield N.R. 2012. Pdl1 is a putative lipase that enhances Photorhabdus Toxin Complex secretion. PLOS Pathogens. 8(5): e1002692.
5. Beeton M. L., Daha M. R., El Shanawany T., Jolles S. R., Kotecha S. & Spiller O.B. 2012. Serum killing of Ureaplasma parvum shows serovar-determined susceptibility for normal individuals and Common Variable Immuno-Deficiency patients. Immunobiology. 217 (2): 187-94
6. Beeton M. L., Maxwell N. C. Spiller O. B., Davies P. L. & Kotecha S. 2011. Risk of postnatal infection in the development of CLD. Eur Respir J. 37 (6): 1424-1430
7. Davies P. L., Beeton M. L., Spiller O. B., Maxwell N. C., Remold-O’Donnell E, and Kotecha S. 2010. Anti-protease defence: The Role of Alpha 1-antitrypsin and Serpin B1 in the development of Chronic Lung Disease of Prematurity. Thorax. 65(3): 246-51
8. Beeton M. L., Chalker V. J, Kotecha S. and Spiller O. B. 2009. Comparison of full gyrA, gyrB, parC, and parE gene sequences between all members of Ureaplasma parvum and urealyticum species to separate polymorphism from true quinolone resistance determinants. J Antimicrob & Chemother. 64(3): 529-38
9. Beeton M. L., Chalker V. J, Maxwell N. C, Kotecha S. and Spiller O. B. 2009. Concurrent titration and determination of antibiotic resistance in Ureaplasma species with identification of novel point mutations in genes associated with resistance. Antimicrob Agents Chemother 53(5): 2020-7.
10.Dale C, Beeton M, Harbison C, Jones T, Pontes M. 2006. Isolation, pure culture, and characterization of "Candidatus Arsenophonus arthropodicus," an intracellular secondary endosymbiont from the hippoboscid louse fly Pseudolynchia canariensis. Appl Environ Microbiol. 72(4): 2997-3004.
1. Beeton M. L., Aldrich-Wright. J. & Bolhuis A. The activity of copper(II) complexes against biofilms of methicillin-resistant Staphylococcus aureus. Microbiology and Infection Translational Research Group. 2013 Oral presentation
2. Beeton M. L., Aldrich-Wright. J. & Bolhuis A. The activity of copper(II) complexes against biofilms of methicillin-resistant Staphylococcus aureus. Department of Pharmacy and Pharmacology research day. 2013. Poster presentation
3. Beeton M. L., Vlisidou I., Spiller O. B. and Waterfield N. R. Evasion of the alternative complement pathway by Photorhabdus spp. 13th European Meeting on Complement in Human Disease. 2011. Poster presentation.
4. Beeton M. L., Vlisidou I., Bode H. B. and Waterfield N. R. Antimicrobial activity of stilbene derivatives produced by the insect pathogen Photorhabdus luminescens. South West and South Wales Microbiology Forum 2010. Oral presentation
5. Beeton M. L., Kotecha S. & Spiller O. B. Serovar variations in mechanism and susceptibility for complement killing of Ureaplasma parvum. 12th European meeting on complement in human disease. 2009. Oral presentation
6. Beeton M. L., Chalker V. J., Maxwell N. C., Kotecha S. & Spiller O. B. Identification and characterisation of antibiotic resistance in Ureaplasma spp. Society of General Microbiology spring meeting 2009. Poster presentation.
7. Beeton M. L., Chalker V. J., Maxwell N. C., Kotecha S. & Spiller O. B. Identification of antibiotic resistance in Ureaplasma species and novel mutations associated. Cardiff University - I3 IRG conference 2008. Oral presentation
8. Beeton M. L., Chalker V. J., Maxwell N. C., Kotecha S. & Spiller O. B. A new method for determining antibiotic resistance in Ureaplasma species; A case report on the characterisation of a clinical erythromycin resistance Ureaplasma. Cardiff University – School of Medicine postgraduate day 2007. Poster presentation